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BRAF K601N/BaF3

CBP73386

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I. Introduction
Cell Line Name: BRAF K601N/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Anti-proliferation assay and PD assay
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
 
II. Background
BRAF K601N lies within the protein kinase domain of the Braf protein (UniProt.org). K601N confers a gain of function on Braf, as indicated by increased phosphorylation of MEK and ERK in cell in culture and induction of cell proliferation and cell viability in culture.
 
III. Representative Data

1. WB of BRAF K601N/BaF3

2. Sanger of BRAF K601N/BaF3

Figure 2. Sanger of BRAF K601N/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BRAF K6D1N BaF3 Cells (C12).

 

 

 

 

 

 

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