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首頁(yè) /藥靶模型 /GPCR靶點(diǎn)細胞 /Class B1(Secretin) /CRFR2b/CHO

CRFR2b/CHO

CBP71436

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I. Background
The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
 
II. Introduction
Host Cell:

CHO

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Expressed Gene: CRFR2b
Synonym(s): CRFR2 | CRF-R2 | CRH-R2 | CRF 2 receptor | CRFR2alpha | CRFR2beta
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+5ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for CRFR2b

Transducer: Gs
 
Ⅲ. Description of Host Cell Line
Organism: Hamster
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. HTRF cAMP Assay With CRFR2b CHO Cells(C11).

 

 

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