BAFF CHO
CBP74286
I. Background | |
BAFF(B 細胞激活因子,B Cell Activating Factor),也稱(chēng)為 TNFSF13B(Tumor Necrosis Factor Superfamily Member 13B)或 BLyS(B Lymphocyte Stimulator),是一種屬 于腫瘤壞死因子(TNF)超家族的細胞因子。BAFF 主要由單核細胞、巨噬細胞、樹(shù)突狀 細胞和某些基質(zhì)細胞產(chǎn)生,在 B 細胞的存活、增殖和成熟中起關(guān)鍵作用。 BAFF 通過(guò)與受體結合,調節 B 細胞的發(fā)育和功能,其過(guò)度表達與自身免疫性疾病 (如系統性紅斑狼瘡、類(lèi)風(fēng)濕性關(guān)節炎)密切相關(guān)。BAFF 有三個(gè)主要受體:BAFF-R (BR3)、TACI 和 BCMA。BAFF 與 BAFF-R 結合后,激活 NF-κB 和 PI3K/AKT 通路,促 進(jìn) B 細胞的存活和增殖;TACI 介導 BAFF 和 APRIL(另一種 TNF 家族成員)的信號傳導, 調節 B 細胞的活化和抗體產(chǎn)生;BCMA 主要在漿細胞中表達,介導 BAFF 和 APRIL 的信 號傳導,支持漿細胞的存活。BAFF 的過(guò)度表達與系統性紅斑狼瘡(SLE)、類(lèi)風(fēng)濕性關(guān)節 炎(RA)、干燥綜合等自身免疫性疾病密切相關(guān)。 |
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II. Description | |
BAFF CHO 細胞,在 CHO-K1 細胞的基礎上,穩定表達人 BAFF。 |
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III. Introduction | |
Expressed gene: | BAFF |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | F12K+10%FBS+600 μg/ml Hygromycin B |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Binding Assay,FACS |
IV. Representative Data![]() Figure 1. Recombinant BAFF CHO stably expressing BAFF. ![]() Figure 2. Blocking of BAFF induced TACI Effector Reporter Cell(C32) Activity by BAFF Neutralization Ab with BAFF CHO. |
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![]() Figure 2. Blocking of BAFF induced BAFFR Effector Reporter Cell(C18) Activity by BAFFR Blocking Ab with BAFF CHO. Blocking of BAFF induced BAFFR Effector Reporter Cell(C18) Activity by BAFF Blocking Ab with BAFF CHO. |