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B2M-associated HLA-G/aAPC Cell

CBP74189

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I. Background
      HLA-G基因是一個(gè)功能性的非經(jīng)典I類(lèi)HLA分子,具有經(jīng)典樣HLA抗原結構,位于染色體6p21.3的MHC區,然而與經(jīng)典HLA-I類(lèi)分子相比,HLA-G具有更少的多態(tài)性和更有限的組織分布。β2-微球蛋白(B2M)是細胞膜上組織相容性復合體(MHC,一組編碼人類(lèi)白細胞表面抗原HLA基因群的通稱(chēng))輕鏈結構的組成部分,在正常細胞新陳代謝過(guò)程中與MHC分離后釋放入血。β2-微球蛋白(B2M)分布于幾乎所有有核細胞(尤其在淋巴細胞和單核細胞存在豐富)和生物體液,包括血清、尿液和(關(guān)節)滑液。
 
II. Introduction
Host Cell: N/A
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+3μg/ml puromycin+600μg/ml Hygromycin B
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
 
III. Representative Data

Figure 1. Recombinant B2M-associated HLA-G/aAPC Cell stably expressing B2M.

Figure 2. Recombinant B2M-associated HLA-G/aAPC Cell stably expressing HLA-G.

Figure 3. Dose Response of LILRB1 Blocking Antibody in LILRB1 Effector Reporter Cells (C13) With B2M-associated HLA-G/aAPC Cells (C15).

Figure 4.Dose Response of LILRB2 Blocking Antibody in LILRB2 Effector Reporter Cells (C24) With B2M-associated HLA-G/aAPC Cells(C15).

 

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